Journal: Immunity

Article Title: LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells

doi: 10.1016/j.immuni.2018.06.007

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Article Snippet: A-tailing was performed using 5U Klenow exo - (New England Biolabs), and Illumina TruSeq adaptors were ligated at 16°C overnight using 2000U T4-Ligase (New England Biolabs).

Techniques: Cell Isolation, Recombinant, Electron Microscopy, Staining, SYBR Green Assay, DNA Methylation Assay, Expressing, Labeling, Sample Prep, Methylation, Purification, Mutagenesis, Plasmid Preparation, Transfection

Journal: Molecular cell

Article Title: Transcription Factor-Directed Re-Wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming Toward Trans-differentiation

doi: 10.1016/j.molcel.2019.07.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Ligation was performed in 1.2ml by adding mQ water, T4 DNA ligase buffer to concentration 1X (NEB, B0202), 0.083% TritionX-100, 0.01mg/mL BSA, 2000U/uL T4 DNA Ligase (NEB, M0202) for 4 hr at RT with slow rotation.

Techniques: Recombinant, Transfection, Bicinchoninic Acid Protein Assay, Plasmid Preparation, Software, Microscopy

Journal: Cell Host & Microbe

Article Title: Lassa virus glycoprotein nanoparticles elicit neutralizing antibody responses and protection

doi: 10.1016/j.chom.2022.10.018

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Article Snippet: This was done by mixing 1 μL of expression vector, 1 μL of PCR3 product, and 2 μL of home-made Gibson mix (T5 exonuclease (0.2U; New England Biolabs), Phusion polymerase (12.5U; New England Biolabs), Taq DNA ligase (2000U; New England Biolabs), Gibson reaction buffer (0.5 grams PEG-8000; Sigma Life Sciences), 1 M Tris/ HCl pH 7.5, 1 M MgCl2, 1 M DTT, 100 mM dNTPs, 50 mM NAD + (New England Biolabs), MQ) and incubating the mix for 60 min at 50°C.

Techniques: Virus, Recombinant, Mass Spectrometry, Sequencing, Lysis, Emulsion, Adjuvant, Plasmid Preparation, Molecular Weight, Marker, Electron Microscopy, Luciferase, Software, Chromatography, Random Hexamer Labeling, Luminex, Expressing, Modification, Nano Differential Scanning Fluorimetry